Figure 7

Red-generated duplications to the left and right of lac. Recombination between electroporated linear dsDNA species ACF (left) and GCB (right) generate duplications of chromosomal sequences to the left and right of lac, respectively, through a sequence of recombination events as pictured in Figure 6. (a) In the diagram, the first events are crossovers between the A or B lac segments, with crossovers between the associated F or G flanks pictured as occurring second (b). The same recombinants would also be formed if the orders were reversed, for example, if F recombined before A (not shown). Short arrows indicate the primers initiating DNA synthesis divergently from the D and E segments. (c) In the recombinants, the primers generate PCR products spanning the duplications, of 2765 and 3032 bp, respectively.