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Figure 5 | BMC Molecular Biology

Figure 5

From: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27

Figure 5

Peptide binding studies. A. Schematic of the structure of the Cdc27 protein (upper part) with the sequences of overlapping peptides SpA-SpE shown beneath. B. Immunoblot using anti-myc mAb showing pull-down of Pol1-13Myc protein from fission yeast protein extracts by Sp series peptides. C. Sequences of human peptide set HsA-HsE corresponding exactly to SpA-SpE above. D. Immunoblot using anti-myc mAb showing pull-down of Pol1-13Myc protein from fission yeast protein extracts by Hs series peptides.

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