Figure 1

Cofractionation of Drosophila NuRD homologues in embryonic protein extracts Nuclear protein extracts were prepared from (A) 2–4 h and (B) 0–12 h old embryos, respectively and size-fractionated by FPLC using a Superose 6 column. Proteins from selected fractions were then separated by SDS gel electrophoresis and analysed by Western blotting. (A) During early embryogenesis the long isoform of MBD2/3 (MBD2/3li) cofractionated with MI-2 and MTA-like in fractions 19 to 21 (indicated in red), which correspond to a molecular weight of approx. 1 MDa. The small isoform of MBD2/3 is not expressed at this stage of embryogenesis. (B) During later stages of embryogenesis both isoforms of MBD2/3 cofractionated with MI-2 and MTA-like in fractions 15 to 19 (indicated in red), which correspond to a molecular weight of approx. 2 MDa. The size of marker proteins is shown left and on top, IN indicates input protein.