Figure 4
From: Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator
Direct physical interaction of Bel1/Tas with p300 determined by co-immunoprecipitation. Bel1/Tas binds to p300 in vitro and in vivo. After transfection of 293T cells with expression plasmids pbel1s and p300FL, cells were labeled with [35S]-Methionine plus [35S]-Cysteine. Cellular extracts were precipitated, separated by SDS-PAGE and exposed. Protein p300FL is marked by bold arrowhead (lanes 1, 3, and 6). In the control, pbel1s was omitted (lane 4). Arrowheads indicate the following protein size markers (M, lanes 2 and 5): myosin of apparent molecular mass of 236, phosphorylase b of 97, and BSA of 66 kDa.