Figure 1

Effect of serum and TPA on gene expression in WT and DN-MKL1 cell lines. Serum starved cells were treated with new born calf serum (20%) or TPA (100 ng/ml) for 1 hour and relative mRNA levels for c-fos and jun B were measured by quantitative real-time PCR using the SYBR green method. Data are represented as the relative fold activation ± the standard deviation of the induced cells compared to the serum starved WT cells.