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Figure 7 | BMC Molecular Biology

Figure 7

From: The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner

Figure 7

a) Reciprocal co-immunoprecipitation of p68 and p72 with HDAC1. Myc-tagged p68 and p72 were immunoprecipitated from nuclear extracts of 293 cells expressing these proteins using a myc epitope-specific antibody and associated HDAC1 was detected by western blotting with an HDAC1-specific antibody. 293 cells, which had not been transfected, were used as control. b) Co-immunopreciptiation of p68/p72 deletion derivatives with HDAC1. HDAC1 was immunoprecipitated from nuclear extracts of 293 cells expressing GAL4-tagged p68 and p72 deletion derivatives, which encompass residues 1–478 and 1–474 of p68 and p72 respectively. Associated p68/p72 were detected by western blotting with a GAL4-specific antibody. 293 cells, which had not been transfected, were used as control. NE-nuclear extract, IP-immunopreciptiation. Molecular weight markers (in kDa) are indicated.

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