Functional interaction between RNase III and the Escherichia coli ribosome

BMC Molecular Biology

Table 1 Association of RNase III with ribosomes and ribosomal subunits.

[³⁵S]RNase III (30 000 dpm) was incubated with 1 A₂₆₀ unit 70 S ribosomes or with the same amount of isolated subunits followed by separation of ribosomes from unbound radioactivity by a Sephacryl S400 spin column using NH₄Cl concentration as indicated. Ribosome-bound RNase III was determined by TCA precipitation and counting the precipitate for radioactivity. The results are shown in dpm. In the control experiments 2 A₂₆₀ units of poly(U) was added to the incubation mixture. The control with [³⁵S]RNase III alone is also shown. The data presented here are average results of 4 parallel experiments.

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