Clones | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|
pcDNA/PGK-neo* | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
pcDNA-redb/PGK-neo* | 0 | 4 | 20 | 0 | 7 | 0 | 0 | 0 | 11 | 0 | 6 | 5 |
- ES cells were electroporated with pcDNA derivatives containing the filled-in neo gene (neo*) under a PGK promoter without or with redβ cloned under the CMV promoter. Stable colonies were isolated by hygromycin resistance, conveyed by the pcDNA vector, and 12 randomly picked clones were taken for each of the 2 vectors. These colonies were expanded separately and electroporated with a 70 nt repairing oligo for the neo* gene, followed by plating and selection for G418 resistance. The numbers shown are the numbers of colonies that arose for each of the 2 × 12 clones.
