Figure 2

Relationship between oligo length and ssOR efficiency. The graph shows relative ssOR efficiencies, scored as kanamycin resistance colonies normalized to ampicillin resistant colonies for oligos of the indicated lengths centered around the Nco1 site in the neo gene. All oligos were lagging and were co-electroporated with pGKneoΔ into single batches of competent cells carrying either pBAD24 vector (diamonds), pBAD24-recT (squares) or pBAD24-redβ (triangles) that had been cultured with arabinose to induce expression from the pBAD promoter. Results are from one experiment and a repetition using 130 and 160 mers synthesized a second time gave the same profile.