Figure 4From: Aggregation and retention of human urokinase type plasminogen activator in the yeast endoplasmic reticulumDistribution of uPA between the supernatant (S) and pellet (P) fractions of cell lysates. Western blot analysis of uPA. The lysates of the following transformants were fractionated by centrifugation: DL/pAM226 (uPA), DL/pAM410 (uPA-C), DLpNR4 (uPA-KDEL) and DL/pNR5 (uPA-C-KDEL). Pellets were dissolved in a 5-fold volume of starting lysate.Back to article page