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Table 1 Structure and sequences of c-myc antisense and control oligonucleotides

From: Expression of c-myc is not critical for cell proliferation in established human leukemia lines

Methylphosphonodiester / phosphodiester chimeric oligodeoxynucleotides
5'F-MD685AS 5'–Fluos-C/T/G/C/T/G/T-C-G-T-T-G-A-G-A/G/G/G/T/A–3'
AS targeting 1147–1166  
5'F-MD586iAS 5'–Fluos-A/T/G/G/G/A-G-A-G-T-T-G-C-T/G/T/C/G/T/C–3'
Inverted AS control  
5'F-MD586S 5'–Fluos-T/A/C/C/C/T-C-T-C-A-A-C-G-A/C/A/G/C/A/G–3'
Sense 1147–1166 control  
5'F-MD757AS 5'–Fluos-C/T/G/C/T/G/T/C-G-T-T-G-A/G/A/G/G/G/T/A–3'
AS targeting 1147–1166  
CpG oligodeoxynucleotide 5-mer
CGTTG 5 '–Fluos-C-G-T-T-G-ProOH–3'
CpG motif present in 1147–1166 AS and 559–573 AS  
Morpholino oligonucleotide analogues
M28AS 5'–Fluos-GGC AUC GUC GCG GGA GGC UGC UGG AGC G–3'
AS targeting from within Intron 1 to 563  
M28NS 5'–Fluos-CAG GCC UUG UGG ACG GCG GUU CGC GAG G–3'
Nonsense control  
M28S 5'–Fluos-CGC UCC AGC AGC CUC CCG CGA CGA UGC C–3'
Sense from within Intron 1 to 563 control  
  1. / = Methylphosphonodiester link; - = phosphodiester link; Fluos = fluorescein label; -ProOH = 3-hydroxypropyl phosphate group Numbering refers to base positions in c-myc mRNA (HSMYC1, GenBank Accession Number V00568)