Plasmid | Gene targeting | Random integration | GT/RI |
---|
ITR-lys2URA3lys2-ITR | 31.0 ± 11.3 (7025) | 0.62 ± 0.37 (487) | 50.2 |
lys2URA3lys2
| 35.4 ± 10.4 (6960) | 1.82 ± 0.41 (3420)* | 19.4 |
- Yeast was transformed with Pvu II- or Xba I-restricted pAAVLUL as reported in Materials and Methods. Gene targeting and random integration events are reported as number of URA3+lys2-colonies /103 transformants per μg of plasmid DNA and URA3+LYS2+colonies /103 transformants per μg of plasmid DNA, respectively. The number of transformants per μg of DNA was determined by transformation with 1 μg of episomal plasmid DNA. Results are the mean of four independent experiments ± standard deviation. The number of total colonies is indicated in parentheses. Statistical analysis was performed using Student’s t- test. *p = 0.0048 vs ITR-lys2URA3lys2-ITR.