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Figure 4 | BMC Molecular Biology

Figure 4

From: Inverted terminal repeats of adeno-associated virus decrease random integration of a gene targeting fragment in Saccharomyces cerevisiae

Figure 4

Expression of Rep proteins and random integration of an AAV vector without homology with the yeast genome. A) Western blot analysis of total cell lysate from yeast cells not expressing (lane 1) and expressing Rep proteins (lane 2) and transformed with pAAVPokURA carrying the ITRs. PGK3 antibody is used as loading control. B) Southern blot of genomic DNA of clones derived from transformation of Rep expressing yeast strain with pAAVPokURA. Lanes 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23: genomic DNA not restricted with Ase I; lane 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24: genomic DNA digested with Ase I that does not cut in the sequence between the ITRs containing Pok and URA3.

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BMC Molecular Biology

ISSN: 1471-2199

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