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Table 2 qPCR parameters describing the standard curve for each primer pair on eleven glycogenes and three reference genes

From: Expression of GBGT1 is epigenetically regulated by DNA methylation in ovarian cancer cells

Gene Slope Intercept Efficiency in % R2 Dilution range
UGCG −3.1875 21.58 105.9 0.999 10 ng-100 ng
GBA −3.2175 24.48 104.6 0.998 10 pg-100 ng
B4GALT6* −3.1905 29.01 105.8 0.998 5 pg-50 ng
GLB1 −3.1640 24.29 107.0 0.991 1 pg-10 ng
A4GALT −3.3425 28.63 99.1 0.998 10 pg-100 ng
GLA −3.2025 25.75 105.2 0.998 10 pg-100 ng
B3GALNT1 −3.2425 27.92 103.4 0.999 10 pg-100 ng
HEXA −3.1575 23.92 107.3 0.999 10 pg-100 ng
HEXB −3.2300 22.51 104.0 0.999 10 pg-100 ng
GBGT1 −3.395 32.69 97.0 0.995 100 pg-100 ng
NAGA −3.152 28.36 107.0 0.999 100 pg-100 ng
HSPCB* −3.250 20.09 103.1 0.998 1 pg-100 ng
YWHAZ* −3.294 20.35 101.2 0.998 1 pg-100 ng
SDHA* −3.194 24.64 105.6 0.994 1 pg-100 ng
  1. *Standard curve was established previously [32].