Table 2 qPCR parameters describing the standard curve for each primer pair on eleven glycogenes and three reference genes
From: Expression of GBGT1 is epigenetically regulated by DNA methylation in ovarian cancer cells
Gene | Slope | Intercept | Efficiency in % | R2 | Dilution range |
|---|---|---|---|---|---|
UGCG | −3.1875 | 21.58 | 105.9 | 0.999 | 10 ng-100 ng |
GBA | −3.2175 | 24.48 | 104.6 | 0.998 | 10 pg-100 ng |
B4GALT6* | −3.1905 | 29.01 | 105.8 | 0.998 | 5 pg-50 ng |
GLB1 | −3.1640 | 24.29 | 107.0 | 0.991 | 1 pg-10 ng |
A4GALT | −3.3425 | 28.63 | 99.1 | 0.998 | 10 pg-100 ng |
GLA | −3.2025 | 25.75 | 105.2 | 0.998 | 10 pg-100 ng |
B3GALNT1 | −3.2425 | 27.92 | 103.4 | 0.999 | 10 pg-100 ng |
HEXA | −3.1575 | 23.92 | 107.3 | 0.999 | 10 pg-100 ng |
HEXB | −3.2300 | 22.51 | 104.0 | 0.999 | 10 pg-100 ng |
GBGT1 | −3.395 | 32.69 | 97.0 | 0.995 | 100 pg-100 ng |
NAGA | −3.152 | 28.36 | 107.0 | 0.999 | 100 pg-100 ng |
HSPCB* | −3.250 | 20.09 | 103.1 | 0.998 | 1 pg-100 ng |
YWHAZ* | −3.294 | 20.35 | 101.2 | 0.998 | 1 pg-100 ng |
SDHA* | −3.194 | 24.64 | 105.6 | 0.994 | 1 pg-100 ng |