Table 1 qPCR primers
From: Expression of GBGT1 is epigenetically regulated by DNA methylation in ovarian cancer cells
Gene | Accession number | Gene Name | Forward Primer 5’-3’ | Reverse Primer 5’-3’ | Amplicon size in bp |
|---|---|---|---|---|---|
A4GALT | NM_017436 | α1,4-galactosyl transferase | CGCTGGAGCTAGAGATGGATTTGC | AGCCGACCTTCTTTGCCAACAC | 78 |
GLA | NM_000169 | α galactosidase A precursor | TCTAATGACCTCCGACACATCAGC | ACACTTCAAAGTTGTCTCCCTGTC | 130 |
HEXA | NM_000520.4 | β hexosaminidase α chain precursor | TTTGTCACACTTCCGCTGTGAG | ACTCCTGCTCACAGAAGCCTAC | 80 |
GBA | NM_001171812 | glucosidase, β, acid | ACAGCCACAGCATCATCACGAAC | TGGGACTGTCGACAAAGTTACGC | 117 |
UGCG | NM_003358 | UDP-glucose ceramide glucosyltransferase | TGTGTTGGATCAAGCAGGAGGAC | AACCTCCAACCTCGGTCAGCTATC | 96 |
B4GALT6 | NM_004775 | UDP-Gal:βGlcNAcβ1,4- galactosyltransferase, polypeptide 6 | AGGAGGTCCCTATGGCACTAAC | TCTCTACAGACAGGCCCATTAGTC | 89 |
GLB1 | NM_001079811 | galactosidase, β1, transcript variant 1 | TGGCCAGCCATTTCGCTACATC | TGAAAGTTCCAGGGCACATACGTC | 135 |
HEXB | NM_000521 | β-hexosaminidase β chain precursor | GCAAGTGCTGTTGGTGAGAGAC | GTTGTGCAGCTATTCCACGTTCG | 118 |
GBGT1 | NM_021996.4 | Globoside α-1,3-N-acetylgalactosaminyltransferase 1 | GCACAAGCTTCAGTGTCCTGTG | TGGCTTCTCCCTCTTGTAGTGC | 122 |
B3GALNT1 | NM_033169 | β1,3-N-acetylgalactosaminyl transferase 1 | TGCTCTATCACGTGGTGCTCTC | ACGCGAGCCGAAGGTTCTTTAC | 62 |
NAGA | NM_000262.2 | N-acetylgalactosaminidase, α | AGCTTCCAGAGCCCAACACATAC | ACATGTCCCAGCAAGAGCACTG | 113 |
HSPCB | NM_007355 | Heat shock protein 90 kDa α | TCTGGGTATCGGAAAGCAAGCC | GTGCACTTCCTCAGGCATCTTG | 80 |
YWHAZ | NM_001135702 | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypetide | ACTTTTGGTACATTGTGGCTTCAA | CCGCCAGGACAAACCAGTAT | 94 |
SDHA | NM_004168 | Succinate dehydrogenase complex, subunit A | TGGGAACAAGAGGGCATCTG | CCACCACTGCATCAAATTCATG | 86 |