Elevated HPA-staining in A2780 cells treated with 5-Aza. (A) Flow cytometry data (representative of three independent experiments) showing a rightwards peak shift (black solid line) after 48 h and 72 h of 5-Aza-treatment relative to the mock control (grey line). The unstained control is shown by the dashed line. (B) Plot illustrates the increase in HPA-staining manifested as the increase in the mean fluorescence intensity (MFI) relative to the mock controls as a function of time after 5-Aza -treatment. Data presented as the mean ± SD of three independent experiments.