Figure 7

Linking of FaPKS6 to the formation of fusaristatin A. A) Extracted ion chromatogram, at 657.4222 Scan Frag = 365.0 V, for the wild type and three ΔFaPKS6 transformants grown on YES medium. The two confirmed PKS6 replacement strains T3 and T4 have lost the ability to produce fusaristatin A whereas the ΔFaPKS6-T5 strain (failed replacement) retains the ability. B) Mass spectrum (m/z plot) for the peak at 11.949 minutes and identification of the molecular ion [M-H]⁻ 657.4248 and [M-H + HCOOH]⁻ 703.4294 adduct. C) MS/MS based fragmentation in negative and positive mode, using a CID of 20.0 eV: Top panel shows fragmentation of the [M-H]⁻ mass ion and the bottom panel shows fragmentation of the [M + H]⁺ ion.