Figure 3

Determination of most stable gene and optimal number of RGs. In the third step of RG selection, the Normfinder algorithm was used on the genes remaining following the first and second eliminations. Gene ranking according to stability through VEGF treatment of ASCs (A) and BMSCs (B). Accumulated SDs indicate the best number of RGs for ASCs (C) and BMSCs (D). The intergroup treatment variation as calculated by Normfinder during Step 3 of the RG selection process (E). The shaded histograms present the single most stable reference gene. ASCs; adipose-derived stromal cells, BMSC; bone marrow-derived stromal cells, SD; standard deviation. N = 8.