Figure 1

C2C12 lineage-specific miRNA expression. C2C12 cells were treated with (diamonds) or without (circles) 300 ng/ml BMP2 for 6 days, during which chromatin and RNA were harvested in parallel at d0, d1, d3 and d6. A) Pol-II enrichment [27] at regions associated with miRNA genes miR-21 (RefSeq Accession NR_029738; enriched region from −1492 to +1661 bp), miR-34b/c (RefSeq Accessions NR_029655 and NR_029654; enriched regions from −287 to +236 bp (miR-34b) and −822 to −299 bp (miR-34c)), miR-99b (RefSeq Accession NR_029536; enriched region from −1113 to −399 bp), miR-365-2 (RefSeq Accession NR_029959; enriched region from +187 to +1282 bp), miR-378 (RefSeq Accession NR_029879.1; enriched region from −2068 to −130 bp) and miR-675 (RefSeq Accession NR_030416.1; enriched region from −1055 to −235 bp) as determined by Pol-II ChIP-on-chip analysis of single biological samples [27]. B) Mature miRNA levels of miR-365 (3p) and miR-378 (3p) as determined by real-time PCR and expressed as a percentage of the control small, non-coding RNA snoRNA202. The mean values +/− SD from duplicate measurements are shown for all data points.