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Figure 1 | BMC Molecular Biology

Figure 1

From: UtroUp is a novel six zinc finger artificial transcription factor that recognises 18 base pairs of the utrophin promoter and efficiently drives utrophin upregulation

Figure 1

A graphical representation of the human and mouse utrophin promoters and the amino acid sequences of the six zinc finger UtroUp peptide (GenBank JQ073900). A: On the left is shown an alignment of DNA sequences of the human and mouse utrophin promoter “A”. The UtroUp DNA target sequence, which is 18 base pairs long, is underlined. The DNA target sequence is 100% conserved between humans and mice. On the right is shown the 18-base-pair UtroUp DNA target sequence. The portion of the Jazz DNA target sequence is indicated in italics. B: The amino acid sequence of the synthetic six zinc finger DNA-binding domain of the UtroUp peptide. Each zinc finger domain in the tandem array is underlined and numbered. The amino acid residues in the α-helix (−1, +3 and +6), which are key for the DNA-binding specificity, are indicated in bold. The zinc finger domains 4, 5 and 6 are inherited by the Jazz peptide. C: A schematic representation of the bacterially produced UtroUp peptide fused to glutathione S-transferase (GST).

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