Figure 6

Nrf2 is not related to ATF4 expression. A shows ATF4 expression after Nrf2 knockdown. The ATF4 mRNA levels were normalized by the β-actin mRNA level at each point (ATF4/β-actin). The ATF4/β-actin values were calculated relative to the expression level at 0 h (the time of knockdown), which was set equal to 1. Bars indicate the standard deviation of independent triplicate measurements. * indicates that there is a significant difference (*: P < 0.05, **: P < 0.005). B shows western blot analyses using an antibody against ATF4. + and – indicate the presence and absence, respectively, of glucose, control RNA, and Nrf2 siRNA. All RNA or protein samples were derived from HepG2 cells at 24 h after knockdown.