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Table 4 Transcripts at lower levels in stationary (high-toxin-producing) as compared to late-exponential (low-toxin-producing) phase in Pseudo-nitzschia multiseries ( Ps-n ) as determined by cDNA microarray analysis

From: Gene expression studies for the analysis of domoic acid production in the marine diatom Pseudo-nitzschia multiseries

   Fold changea  
Stationary versus late-exponential phase
Ps-n NR Identifier JGI Ps-n Genome Hit Non-axenic Non-axenic Axenic Predicted gene product
  Scaffold:Start-End Expt. 1 Expt. 2 Expt.  
PSN0100 32:397085-398987 0.34 ± 0.01 0.33 ± 0.05 0.39 ± 0.01 Pyrophosphate-dependent phosphofructokinase (PFK)
PSN0060 133:16659-18505 0.20± 0.02 0.16 ± 0.03 0.44 ± 0.04 Predicted protein with signal or transit peptide
PSN0048 188:179178-180986 0.36± 0.08 0.25 ± 0.06 0.52 ± 0.05 Predicted protein with signal or transit peptide
PSN0080 461:123066-124152 0.32 ± 0.02 0.33 ± 0.03 0.57 ± 0.07 Predicted protein with mitochondrial transit peptide
135E4 1441:17433-18891 0.17 ± 0.00 0.19 ± 0.02 0.45 ± 0.02 Predicted protein
165G9 8:175639-176910 0.37 ± 0.02 0.37 ± 0.02 0.62 ± 0.04 Tetratricopeptide repeat protein
135H6b 214:78592-7971 0.41 ± 0.00 0.22 ± 0.02 0.59 ± 0.00 Fucoxanthin-chlorophyll a-c binding protein,
      chloroplastic (FCP)
  1. aThe fold-change data presented are the average of all of the cDNA clones that were printed on the array for each transcript. The number of cDNA clones for each transcript was: PSN0100 (2), PSN0060 (5), PSN0048 (5), PSN0080 (3), 135E4 (1), 165G9 (1), 135H6 (1), and each clone was printed twice.
  2. b135H6 was included as a gene of interest in RT-qPCR analysis, below, although it did not meet our fold-change cut-off for the original FDR microarray analysis (yet, all LFDRs were <10%). Please see Methods for statistical analysis, and Additional file1 for FDR and LDFR data.