Figure 4

Nuclear NF-κB proteins and expression of miR-155 in human B-lymphoma cell lines. A panel of nine B-lymphoma cell lines was analyzed for (A) mature miR-155 expression by qPCR and (B) nuclear p65, REL, and PARP (as a normalizing control) by Western blotting. Included are cell lines known to have low NF-κB activity (Ramos, Daudi, SUDHL4, Pfeiffer, BL41, and BJAB) and cell lines known to have high NF-κB activity (IB4, KMH2, and L428) (see text and Methods). miR-155 expression values are relative to Ramos cells (1.0) and error bars indicate +/- SE.