|
attB-F
|
GTCATTAATCGCCATTCAGGCTGCGCA
|
To amplify 400 bp attB site in AseI of pEGFP-N1
|
|
attB-R
|
GTCATTAATCTCGGCCTCGACTCTAG
|
|
attL-F
|
GGCGAGAAAGGAAGGGAAG
|
To detect the attL site, 400 bp
|
|
attL-R
|
ATTAACCCTCACTAAAGGGA
|
|
attR-F
|
TCAAAGTAAACGACATGGTG
|
To detect the attR site,520 bp
|
|
attR-R
|
CCAACCGCTGTTTGGTCTGC
|
|
IV-R1
|
GCTTATAGATACCGTAGACAT
|
TAIL-PCR to identify integration site
|
|
IV-R2
|
TCCCGTGCTCACCGTGACC
|
|
IV-R3
|
ATCAACTACCGCCACCTCG
|
|
IV-F2
|
CGCCACCTCTGACTTGAGCG
| |
|
5113-LF
|
GTTGGGATTTGGCTCCAGCC
|
Use with IV-R1 for determination of 5′ end of 5113 pseudo attP site
|
|
5113-RR
|
TCTTAACACCCCATTGTTCTC
|
Use with IV-F2 for determination of 3′ end of 5113 pseudo attP site
|
|
5156-LF
|
GCCTTAATGAGGGCCAGAGC
|
Use with IV-R1 for determination of 5′ end of 5156 pseudo attP site
|
|
5156-RR
|
GTTGACCCATTTTCCTGCTCTTCG
|
Use with IV-F2 for determination of 3′ end of 5156 pseudo attP site
|
|
1015-LF
|
GTCCTGCCCTGACCCTTTGG
|
Use with IV-R1 for determination of 5′ end of 1015 pseudo attP site
|
|
1015-RR
|
GCCAACCTCCAAGTTGTTGG
|
Use with IV-F2 for determination of 3′ end of 1015 pseudo attP site
|
|
2015-LF
|
GCAAGGGGTT TTGAAACTGC
|
Use with IV-R1 for determination of 5′ end of 2015 pseudo attP site
|
|
2015-RR
|
AAAAGTGCCTCAGAACACCAC
|
Use with IV-F2 for determination of 3′ end of 2015 pseudo attP site
|
|
5113-pF
|
TCAAAGTAAACGACATGGTGGAGTGTAGCAGTTCCTAGGC
|
ABI-REC cloning of 5113 pseudo attP site in pBCPB+ plasmid
|
|
5113-pR
|
ATTAACCCTCACTAAAGGGATCTTAACACCCCATTGTTCTC
|
|
5156-pF
|
TCAAAGTAAACGACATGGTGGACCTCTGCTGTGCCCAGAG
|
ABI-REC cloning of 5156 pseudo attP site in pBCPB+ plasmid
|
|
5156-pR
|
ATTAACCCTCACTAAAGGGACCAACCACTGGCTTCCCTCG
|
|
1015-pF
|
TCAAAGTAAACGACATGGTGACCCCCACCCTGAGCTACTC
|
ABI-REC cloning of 1015 pseudo attP site in pBCPB+ plasmid
|
|
1015-pR
|
ATTAACCCTCACTAAAGGGAACTCCCCAGCAACCAGCTTC
|
|
2015-pF
|
TCAAAGTAAACGACATGGTGATGAGACAAGAGGGACCAGG
|
ABI-REC cloning of 2015 pseudo attP site in pBCPB+ plasmid
|
|
2015-pR
|
ATTAACCCTCACTAAAGGGAAAAAGTGCCTCAGAACACCAC
|
|
Pseudo-P1R
|
CACCATGTCGTTTACTTTGA
|
ABI-REC cloning of pig pseudo site
|
|
BCPB-S
|
GCTGCAAGGCGATTAAGTTGGG
|
Sequencing primer
|
|
EGFP-QF
|
TGAACCGCATCGAGCTGAAGGG
|
Real-time PCR primers, 130 bp
|
|
EGFP-QR
|
ACCTTGATGCCGTTCTTCTGCTTG
|
|
TFRC-QF
|
GAGACAGAAACTTTCGAAGC
|
Real-time PCR primers, 81 bp
|
|
TFRC-QR
|
GAAGTCTGTGGTATCCAATCC
|
