A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector

Table 1 Plasmids encoding functional modules currently available on the toolkit

Plasmids based on the pDONR P4-P1R vector contain inserts that will be placed at the 5′ flank of the cDNA and will encode the N-terminal module of the fusion protein. On the other hand, those based on pDONR P2R-P3 will transfer their insert to a position flanking the 3′-end of the cDNA, and will add a C-terminal module to the fusion protein in case they encode a peptide.

ISSN: 1471-2199