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Figure 6 | BMC Molecular Biology

Figure 6

From: Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

Figure 6

Evaluation of the TsoI-resistance of plasmids isolated from the same culture before and after TsoI synthesis induction. Lane M, DNA standard (Gene Ruler™ Ladder Mix (Thermo Fisher Scientific (Fermentas); selected bands marked); lanes 1 and 4, uncleaved pET21NS-TsoIRM plasmid DNA; lanes 2 and 5, pET21NS-TsoIRM after incubation with TsoI; lanes 3 and 6, pET21NS-TsoIRM supplemented with λ DNA and then incubated with TsoI.

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BMC Molecular Biology

ISSN: 1471-2199

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