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Figure 1 | BMC Molecular Biology

Figure 1

From: Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

Figure 1

Restriction analysis of large recombinant plasmids isolated during the biochemical selection of T. scotoductus library. Patterns of DNA fragments resulting from the HindIII cleavage of recombinant plasmids, isolated after two rounds of biochemical selection using TsoI, visualized on electrophoretic agarose/Tris-borate-EDTA (TBE) gel (1%), stained with ethidium bromide (EtBr). Arrows show cloned fragments of 1.75 and 2 kb in size, which originate from T. scotoductus and are common for all TsoI-selected plasmids examined. Lane M - DNA standards (Gene Ruler™ Ladder Mix (Thermo Fisher Scientific (Fermentas); selected bands marked).

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BMC Molecular Biology

ISSN: 1471-2199

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