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Table 1 SCE levels and EGFP expression

From: Escherichia coli RecG functionally suppresses human Bloom syndrome phenotypes

Cells SCE/Chr EGFP % EGFP+ HPF Xomes
HeLa 0.09 1.0 0 21 605
HeLa: RecG fuse 0.08 42.6 98 32 835
BLM+ 0.10 1.0 0 22 863
BLM+: RecG 2a 0.10 49.3 100 30 999
BLM-: cDNA 0.28 1.0 0 26 1625
BLM-: RecG 2a high 0.33 148 93 33 1861
BLM-: RecG fuse high 0.34 29.8 87 26 1149
BLM-: RecG 2a low 0.68 12.4 92 36 2017
BLM-: EGFP 1.02 27.3 57 48 3013
BLM-: empty vector 1.05 1.0 0 25 1354
BLM- 0.98 1.0 0 16 883
BLM- FC: RecG fuse 0.70 6.4 100 55 1981
BLM- FC 0.91 1.0 0 49 2077
BLM-: RecG fuse med 0.43 30.6 87 29 1925
BLM-: lost RecG fuse 0.86 8.96 1 25 1316
  1. Cells: The stable cell line being investigated.
  2. SCE/Chr: Median number of sister chromatid exchanges observed per chromosome (sister chromatid pair).
  3. EGFP: Relative levels of fluorescence for cells expressing RecG and/or EGFP, relative to cells not expressing EGFP.
  4. % EGFP+: The fraction of cells in a population with above-background green fluorescence due to expression of EGFP.
  5. HPF: The number of high powered microscope fields scored.
  6. Xomes: The total number of sister chromatid pairs scored for exchanges.