Binding of tritiated ponasterone A to in vitro- translated EcRs and USP of P. xylostella , respectively. Expressed proteins were incubated with 10nM tritiated PonA, and filtered through a nitrocellulose membrane. T, total binding; N, non-specific binding. The data were analyzed by one-way ANOVA and Tukey’s test using GraghPad InStat (GraphPad software Inc., San Digeo CA, USA). “*” and “**” denote that the total binding are significantly different with non-specific binding at α=0.05 and α=0.01 level, respectively.