Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

Table 5 Validation of reference gene suitability as an internal control based on the correlation of amplification efficiency between reference and target genes in fluconazole-treated C. glabrata cells

Gene	CDR1	PDH1	PDR1	SNQ2	YOR1	ERG2	ERG3	ERG4	ERG10	ERG11
ACT1	0.056	0.072	0.061	0.019	0.033	0.046	0.034	0.095	0.054	0.043
PGK1	0.018	0.049	0.014	0.059	0.094	0.057	0.032	0.024	0.046	0.028
RDN5.8	0.084	0.065	0.014	0.021	0.014	0.086	0.060	0.060	0.029	0.061
RDN18	0.187	0.315	0.304	0.262	0.210	0.836	0.689	0.689	0.778	0.688
UBC7	0.015	0.051	0.012	0.062	0.096	0.077	0.052	0.044	0.066	0.048
UBC13	0.069	0.051	0.028	0.035	0	0.079	0.068	0.068	0.022	0.069

Each value is the slope m of the line (Y = mx + b) of the validation experiment and reflects the correlation of reference gene and target gene amplification efficiencies.
An absolute slope value <;0.1 is generally used as a criterion for passing a validation experiment, as it indicates that the amplification efficiency is approximately equal between the reference and target genes.
The slope m = 0 indicates that the efficiencies of the two PCR reactions are equal.

ISSN: 1471-2199