Figure 5

Endogenous VDR target gene mRNA expression in PIM-1 overexpressing human keratinocytes. With PIM-1 overexpression the levels of CYP24A1, PIM-1, and OPN are increased after normalization with the housekeeping gene ANXA1. Results are shown as fold change of PIM-1 overexpression relative to cells transfected with the empty vector. The significant differences of PIM-1 overexpression compared to the empty control vector samples was considered as true by the low p-values. Error bars represent the SEM of n = 3 values.