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Table 3 Oligonucleotides used for EMSA

From: Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter

OLIGONUCLEOTIDE SEQUENCE
Sp1-A Sp1-As 5-GCTCCGCCGGCACACGCCGCCCCTGGCAGGGGGCCGAGCGC-3
  Sp1-Aas 5-GCGCTCGGCCCCCTGCCAGGGGCGGCGTGTGCCGGCGGAGC-3
Sp1-B Sp1-Bs 5-TGCGCAGCGGGGGTGGGCGCGGGGCGCAGTCGGTGCGGGGCGGGCCGCGGCGGCGGGAGG-3
  Sp1-Bas 5-CCTCCCGCCGCCGCGGCCCGCCCCGCACCGACTGCGCCCCGCGCCCACCCCCGCTGCGCA-3
Sp1-C Sp1-Cs 5-GGCCAGCTAGGGGCGGCCCCCCTCCCGCCCCCCGGCCCAG-3
  Sp1-Cas 5-GTGGGCCGGGGGGCGGGAGGGGGGCCGCCCCTAGCTGGCC-3
M4 M4s 5-GGCCAGCTAGTTTATTCCCCCCTCCCGCCCCCCGGCCCAG-3
  M4as 5-CTGGGCCGGGGGGCGGGAGGGGGGAATAAACTAGCTGGCC-3
M5 M5s 5-GGCCAGCTAGGGGCGGCCCCCCTAAATAACCCCGGCCCAG-3
  M5as 5-CTGGGCCGGGGTTATTTAGGGGGGCCGCCCCTAGCTGGCC-3
M6 M6s 5-GGCCAGCTAGGGGCGGCCCCCCTCCCGCCCCAATTAAAAG-3
  M6as 5-CTTTTAATTGGGGCGGGAGGGGGGCCGCCCCTAGCTGGCC-3
MT MTs 5-GGCCAGCTAGTTTATTCCCCCCTAAATAACCAATTAAAAG-3
  MTas 5-CTTTTAATTGGTTATTTAGGGGGGAATAAACTAGCTGGCC-3
Sp1 Sp1s 5-ATTCGATCGGGGCGGGGCGAGC -3
  Sp1as 3-TAAGCTAGCCCCGCCCCGCTCG-5
Sp1Mut Sp1Muts 5-ATTCGATCGGTTCGGGGCGAG C-3
  Sp1Mutas 3-TAA GCTAGCCAAGCCCCGCTCG-5
  1. Sp1 consensus (Sp1) and Sp1 mutant consensus (Sp1Mut) oligonucleotides were purchased from Santa Cruz Biotechnologies.