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Table 2 Yeast strains and plasmids used in this study.

From: Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene

Strain Relevant genotype Source or reference
Isogenic to W303  
EYL682 MATa mpk1Δ::TRP1 ura3-1 trp1-1 leu2-3,112 ade2-1 ade3-1 his3 can1-100 lys2 (Gal+) Elion lab collection
EYL780 MATa bar1Δ far1Δ ste11-4 ste12Δ::URA3 trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 Elion lab collection
EY957 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) Elion lab collection
EY1298 MATa bar1Δ far1Δ ste11-4 trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 Elion lab collection
EYL4661 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste11Δ::Hygro Elion lab collection
EYL5328 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) pbs2Δ::Hygro This study
EYL5329 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) kss1Δ::HIS3 fus3Δ::Hygro pbs2Δ::Kan This study
EYL5330 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste12Δ::Hygro This study
EYL5331 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) bni1Δ::Hygro This study
EYL5332 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ste7Δ::Hygro pbs2Δ::Kan This study
EYL5333 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cmk2Δ::Hygro This study
EYL5334 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ptk2Δ::Hygro This study
EYL5335 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cnb1Δ::Hygro This study
EYL5336 MATa bar1Δ far1Δ trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 pbs2Δ::Hygro This study
EYL5337 MATa bar1Δ far1Δ trp1-1 his3Δ200 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) FUS1p-HIS3::lys2 cnb1Δ::Hygro This study
EYL5338 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) kss1Δ::HIS3 fus3Δ::Hygro This study
EYL5379 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) ptk2Δ::Hygro cmk2Δ::Kan This study
EYL5380 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) cmk2-tag(3-GFP)Kan This study
EYL5382 MATa bar1Δ trp1-1 his3-11,15 leu2-3,112 ade2-1 ura3-1 can1-100 (GAL+) crz1Δ::Kan This study
S288c deletion strains in Figure 1 are isogenic to S288c BY4741 [46]
BY4741 MATa his3Δ1 ura3Δ0 leu2Δ0 met15-0 [47]
RG2468 ste4Δ::KAN r leu2 ura3 his3 met15 Research Genetics
RG4038 ste5Δ::KAN r leu2 ura3 his3 met15 Research Genetics
RG3439 ste50Δ::KAN r leu2 ura3 his3 met15 Research Genetics
RG5271 ste11Δ::KAN r leu2 ura3 his3 met15 Research Genetics
RG6981 kss1 Δ::KANr leu2 ura3 his3 met15 Research Genetics
JAY408 fus3Δ::KAN r ste5Δ::KAN r [25]
Isogenic to ∑1278b  
L5528 ura3-52, his3::hisG [48]
L5554 ste5::LEU2, ura3-52, leu2::hisG [48]
Plasmids  
pYGU-11ΔN pGAL1-STE11ΔN LEU2 CEN [49]
GAL1 vector pGAL1, LEU2 CEN Elion lab collection
pYEE133 pGAL1-STE12 TRP1 CEN Elion lab collection
GAL1 vector pGAL1, TRP1 CEN Elion lab collection
pOO1 pCMK2-lacZ, URA3, 2 μ [19]
EBL95 pDL1460 pFUS1::ubiY-lacZ, URA3, 2 μ Elion lab collection
[50]
EBL183 PMC1-lacZ, URA3, 2 μ [26]
EBL185 p DM5 FKS2 (-968-+6)-CYC1-lacZ, URA3, 2 μ [26]
EBL201 FKS2 (-928-+1)-CYC1-lacZ, URA3, 2 μ [14]
EBL200 FKS2 (-706-+1)-CYC1-lacZ, URA3, 2 μ [14]
EBL1258 FKS2 (-540--375)-CYC1-lacZ, URA3, 2 μ [15]
EBL513 YKL161c (MLP1)-lacZ, URA3, 2 μ Elion lab collection
EBL514 YIL117c (PRM5)-lacZ, URA3, 2 μ [51]
EBL517 pLGΔ-178 pCYC1-lacZ, URA3, 2 μ [52]