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Table 3 Candidate reference genes stability results using qBaseplus software.

From: Evaluation of suitable reference genes for gene expression studies in bronchoalveolar lavage cells from horses with inflammatory airway disease

Reference target

M value

CV value

GAPDH

0.695

0.274

HPRT

0.830

0.476

RPL32

0.781

0.364

SDHA

0.828

0.388

UBB

0.816

0.347

  1. M values, which is a parameter for the gene stability, and Coefficient of Variation (CV) values, which indicates how stably a gene is expressed, show that GAPDH is the most stably expressed gene.