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Table 3 Antibacterial activity of the immunized haemolymph and the purified mature defensin peptide on gram (-) and gram (+) bacteria.

From: Identification, phylogenetic analysis and expression profile of an anionic insect defensin gene, with antibacterial activity, from bacterial-challenged cotton leafworm, Spodoptera littoralis

Microorganism

Antibacterial activity

(Gram stain)

Immunized haemolymph

Mature purified peptide

 

24 h p.i.

48 h p.i.

24 h p.i.

48 h p.i.

Escherichia coli (-ve)

++

+++

+

+++

Proteus vulgaris (-ve)

+

++

(+)

++

Klebsiella pneumoniae (-ve)

++

+++

+

++

Staphylococcus aureus (+ve)

+++

+++

++

+++

Streptococcus sanguinis (+ve)

+++

+++

+++

+++

  1. (+): Inhibition zone less than 1 mm surrounding the 6 mm paper disk
  2. +: Inhibition less than positive control
  3. ++: Inhibition comparable to positive control
  4. +++: Inhibition more than 10 mg penicillin; inhibition zones of references = 13 ± 1 mm diam.
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BMC Molecular Biology

ISSN: 1471-2199

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