Figure 7

Activity of the bop promoter depending on brz , brb and bat genes expression according to GFP reporter assay. Panel A represents a map of the GFP reporter plasmid. In panels B, C, D, E kinetic of the bop promoter activity during cell growth is shown as GFP fluorescence intensity. The GFP fluorescence is measured in light (red circles) and dark (black squares) for different combinations of the expressed brz, brb and bat genes. OE3095R, transformant containing the reporter plasmid with the OE3095R gene (B); brz/brb/bat, transformant containing the reporter plasmid with the brz, brb, and bat genes (C); brz/bat, transformant containing the reporter plasmid with the brz and bat genes (D); bat, transformant containing the reporter plasmid with the bat gene (E). In each case at least three biological repetitions were performed. The background level of fluorescence in untransformed cells was constant (around 20 units) during cell growth.