Figure 3

Effects of CBD domain insertion in the brb gene on bop transcription. In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD₆₀₀ at which RNA was extracted is given at right. WT R1, wild-type strain R1; brb CBD, brb CBD gene fusion strain. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from the brb CBD mutant corresponded to 67 ± 2% (OD₆₀₀ = 07.-0.8), 70 ± 2% (OD₆₀₀ = 1.1-1.2) of WT in the dark, and 85 ± 3% (OD₆₀₀ = 07.-0.8), 85 ± 2% (OD₆₀₀ = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.