Figure 2

Expression of exons at the tammar TRPC2 locus in adult and pouch young. A. Exons 6-7 (primers 3F & 4R) were expressed in a range of adult tammar tissues whereas expression of Exons 12-14 (primers 4F & 5R) was specific to the VNO. The two bands for Exons 6-7 resulted from the two alternate splice donor sites of Exon 6. "+ RT" and "- RT" denote inclusion and omission of reverse transcriptase, respectively. B. Expression of Exons 12-14 in the VNO was detected weakly in VNO and main olfactory epithelium (MOE) samples at day 0, day 10, day 15 and day 20 postpartum and more strongly from day 34 postpartum. In the adult, expression was highly specific to the VNO, absent in MOE, and only very weakly detected in lung and testis.