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Figure 1 | BMC Molecular Biology

Figure 1

From: Clarifying mammalian RISC assembly in vitro

Figure 1

Recombinant Ago2:5'P -pre-miR-24-1 complex is active against a 5'- but not a 3'-arm target. Cleavage assay results of recombinant Ago2 pre-incubated with 5'-P-pre-miR-24-1 before the addition of radiolabeled 5'-arm (Ta-5) or 3'-arm (Ta-3) target. Predicted sizes of 5'-end cleavage products of 5'- and 3'-arm targets are 17nt and 14nt respectively. Radiolabeled pBR322/MspI (M) was used as size marker.

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