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Table 7 Kinetic parameters for ATP and dATP hydrolysis catalyzed by Dmu RecA.

From: RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation

Substrate

DNA

T (°C)

KM (mM)

kcat (s-1)

kcat/KM (s-1 M-1)

na

ATP

ssDNA

37

0.31 ± 0.009

0.13 ± 0.004

4.3 ± 0.18 × 102

1.1

 

ssDNA and dsDNA

37

0.28 ± 0.009

0.067 ± 0.0021

2.4 ± 0.08 × 102

1.5

dATP

ssDNA

37

0.28 ± 0.007

0.14 ± 0.004

5.0 ± 0.25 × 102

1.0

 

ssDNA and dsDNA

37

0.22 ± 0.007

0.073 ± 0.0023

3.4 ± 0.05 × 102

1.3

  1. a Hill coefficient
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BMC Molecular Biology

ISSN: 1471-2199

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