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Table 1 Experimental design for expression analysis between different P. monodon male broodstock types.

From: A cDNA microarray, UniShrimpChip, for identification of genes relevant to testicular development in the black tiger shrimp (Penaeus monodon)

Experiment

Cy3

Cy5

Gene expression levels in wild-caught broodstock

I

Juveniles

(pooled, n = 114)

West-coast broodstock

(individual, n = 3; GSI* = 1.3, 1.0, 0.7%)

II

Juveniles

(pooled, n = 114)

East-coast broodstock

(individual, n = 3; GSI = 0.9, 0.7, 0.5%)

Gene expression levels in domesticated broodstock

III

Juveniles

(pooled, n = 114)

18-months-old domesticated broodstock (individual, n = 4; GSI = 0.8, 0.6, 0.6, 0.4%)

IV

Juveniles

(pooled, n = 114)

14-months-old domesticated broodstock (individual, n = 4; GSI = 1.0, 0.8, 0.6, 0.4)

V

Juveniles (pooled, n = 114)

10-months-old domesticated broodstock (individual, n = 4; GSI = 1.0, 0.7, 0.6, 0.4)

  1. *GSI is gonadosomatic index calculate as a percentage of testis weight by total body weight