Figure 4
From: Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression
Ctf4 is required for normal cell cycle progression. A. CTF4 knockdown results in reduced mitotic index in larval brain tissue as compared to sibling control. Boxplot of mitotic indices determined from 3rd-instar wandering larva brain squashes. Larvae with the genotype RNAi-Ctf4/Actin-GAL4 (Ctf4 knockdown) yielded a significantly lower ratio of cells progressing through M-phase compared to sibling control (P = 0.004). B-C. Micrographs of mitotic figures from control and RNAi knockdown larval brains. Ctf4 depletion results in premature sister chromatid separation as indicated by triangles (bars represent 10 μm). D. CTF4 knockdown results in S phase delay. Boxplot of S-phase indices of larval brains determined by EdU incorporation showing that significantly more cells are seen in S-phase within Ctf4 knockdown larval brains compared to sibling control (P = 0.009).