Table 4 Strains
From: Recombination Phenotypes of Escherichia coli greA Mutants
MG1655 | Wild type | [43] |
MDS12 | Reduced genome version of MG1655 | [35] |
JW1100 | mfdΔkanFRT | [38] |
JW3369 | greBΔkanFRT | [38] |
TP796 | MDS12 recAΔtet | [44] |
TP798 | MG1655 recBCDΔPtac-gam-bet-exo-cat | [44] |
Strains constructed for this study. All are in the MG1655 background | ||
TP933 | recBCDΔPtac-gam-bet-exo-cat lacIPΔ::bla-P32-aacC-lacZ | TP798 × bpa1,aof2 pcr of pTP1068 |
TP965 | recBCDΔPtac-gam-bet-exo-cat lacIPΔbla'-kan-'lacZ | TP933 × PRO1,2 pcr of Tn903 aph |
TP1195 | greA::tet41 | MG1655/pKM208 × greA9,X pcr of Tn10 tet |
TP1198 | greA-D41N | TP1195/pKM208 × oligo D41N |
TP1201 | recAΔtet | MG1655 × P1•TP796 |
TP1202 | greA-D41N recAΔtet | TP1198 × P1•TP796 |
TP1203 | greA::tet44 | MG1655/pKM208 × greAY,Z pcr of Tn10 |
TP1204 | greA-D41A | TP1195/pKM208 × oligo D41A |
TP1205 | greAΔ | TP1195/pKM208 × oligo greAD |
TP1208 | greA-D41A recAΔtet | TP1204 × P1•TP796 |
TP1209 | greAΔ recAΔtet | TP1205 × P1•TP796 |
TP1213 | greAΔ greBΔtet | TP1205 × P1•TP1199 |
TP1216 | greA-E44K | TP1203/pKM208 × oligo E44K |
TP1218 | greBΔkanFRT | MG1655 × P1•JW3369 |
TP1219 | greA-E44K recAΔtet | TP1216 × P1•TP796 |
TP1220 | greA-E44K greBΔtet | TP1216 × P1•TP1199 |
TP1222 | greBΔFRT | TP1218/pCP20 passaged at 42° |
TP1224 | greA-D41N greBΔkanFRT | TP1198 × P1• JW3369 |
TP1225 | lacIPΔbla'-kan-'lacZ | MG1655 × P1•TP965 |
TP1226 | greA-D41N lacIPΔbla'-kan-'lacZ | TP1198 × P1•TP965 |
TP1227 | greA-D41A lacIPΔbla'-kan-'lacZ | TP1204 × P1•TP965 |
TP1229 | greA-E44K lacIPΔbla'-kan-'lacZ | TP1216 × P1•TP965 |
TP1230 | recBCDΔred-cat greA-D41N | TP1198 × P1•TP798 |
TP1231 | greA-D41N greBΔFRT | TP1224/pCP20 passaged at 41° |
TP1232 | greBΔFRT lacIPΔbla'-kan-'lacZ | TP1222 × P1•TP965 |
TP1233 | greA-D41N greBΔFRT lacIPΔbla'-kan-'lacZ | TP1231 × P1•TP965 |
TP1234 | lacIPΔ::bla-P32 | TP1225/pTP1216 × pTP1232 linear |
TP1235 | greBΔFRT recAΔtet | TP1222 × P1•TP796 |
TP1236 | greA-D41N greBΔFRT recAΔtet | TP1231 × P1•TP796 |
TP1241 | greAΔcat lacIPΔbla'-kan-'lacZ | TP1193 × P1•TP965 |
TP1242 | greAΔ lacIPΔbla'-kan-'lacZ | TP1205 × P1•TP965 |
TP1252 | mfdΔkanFRT | MG1655 × P1•JW1100 |
TP1253 | greA-D41N mfdΔkanFRT | TP1198 × P1•JW1100 |
TP1254 | greA-E44K mfdΔkanFRT | TP1216 × P1•JW1100 |
TP1255 | dksAΔ10::cat | MG1655/pKM208 × cat40,41 pcr of Tn9 |
TP1257 | mfdΔFRT | TP1252/pCP20 passaged at 41° |
TP1258 | greA-D41N mfdΔFRT | TP1253/pCP20 passaged at 41° |
TP1259 | greA-E44K mfdΔFRT | TP1254/pCP20 passaged at 41° |
TP1260 | dksA-D71N/D74N | TP1255/pKM208 × oligo dksNN |
TP1262 | dksA-D71N/D74N recAΔtet | TP1262 × P1•TP796 |
TP1263 | dksAΔ | TP1255/pKM208 × oligo dksAD |
TP1264 | dksAΔ recAΔtet | TP1263 × P1•TP796 |