High throughput nano-liter RT-qPCR to classify soil contamination using a soil arthropod

de Boer, Muriel E; Berg, Sandra; Timmermans, Martijn JTN; den Dunnen, Johan T; van Straalen, Nico M; Ellers, Jacintha; Roelofs, Dick

doi:10.1186/1471-2199-12-11

BMC Molecular Biology

Table 2 Overview of differential qPCR assays and their importance and significances in the statistical analyses

From: High throughput nano-liter RT-qPCR to classify soil contamination using a soil arthropod

#	SSH origin	Collembase ID¹	Gene	PLS²	cadmium³	phenanthrene³	interaction³
1	cadmium	Fcc00008	unknown function	VIP	***	ns	**
2	cadmium	Fcc00101	BCS1	VIP	***	*	ns
3	cadmium	Fcc00344	retinol dehydrogenase 11	VIP	***	*	ns
4	cadmium	Fcc00583	inflammatory response protein 6; viperin;	VIP	***	ns	***
5	cadmium	Fcc01017	Endo-1,3;1,4-beta-D-glucanase precursor	VIP	***	***	***
6	cadmium	Fcc01428	16S ribosomal RNA gene, no sign hits	VIP	***	***	ns
7	cadmium	Fcc01574	glycine receptor, alpha 1	VIP	***	***	ns
8	cadmium	Fcc01821-1	Isopenicillin N synthetase	VIP	***	***	ns
9	cadmium	Fcc01821-2	Isopenicillin N synthetase	VIP	***	***	ns
10	cadmium	Fcc01910	whey acidic protein	VIP	*	**	ns
11	cadmium	Fcc02275	No Significant Hit	VIP	***	**	***
12	cadmium	Fcc03202	No Significant Hit		ns	ns	ns
13	cadmium	Fcc04239	unknown function		ns	*	ns
14	cadmium	Fcc04580	metalloproteinase; nephrosin; astacin 4		ns	ns	ns
15	cadmium	Fcc04660	No Significant Hit	VIP	ns	ns	ns
16	cadmium	Fcc00074	Cephalosporin hydroxylase		***	ns	ns
17	cadmium	Fcc00343	haloacid dehalogenase-like hydrolase	VIP	*	***	ns
18	cadmium	Fcc00966	Short-chain dehydrogenase/reductase 9		ns	ns	ns
19	cadmium	Fcc00343-2	Haloacid dehalogenase like hydrolase	VIP	*	***	ns
20	cadmium	Fcc00002	Catalase		*	ns	ns
21	cadmium	Fcc00343-1	Haloacid dehalogenase like hydrolase	VIP	ns	***	ns
22	cadmium	Fcc00086	LamininA	VIP	**	**	ns
23	cadmium	Fcc03748	unknown function		**	ns	ns
24	cadmium	Fcc00170	α-aminoadipyl-cysteinyl-valine synthetase		***	***	ns
25	phenanthrene	Fcc05839	alcohol dehydrogenase (acceptor)		ns	ns	ns
26	phenanthrene	Fcc05181	immunoglobulin-like, sid-1-like protein 2		ns	ns	ns
27	phenanthrene	Fcc04834	crossveinless-2; Bmper protein		ns	ns	ns
28	phenanthrene	Fcc03839	LMBR1 domain containing 2		ns	ns	ns
29	phenanthrene	Fcc01609	heat shock protein Hsp70g; HSP68		*	***	ns
30	phenanthrene	Fcc06002	ABC transporter; MOAT-D	VIP	ns	***	***
31	phenanthrene	Fcc03172	putative deoxynucleoside kinase	VIP	ns	ns	ns
32	phenanthrene	Fcc03067	Shprh protein		ns	ns	ns
33	phenanthrene	Fcc04344	laminin, beta 2; laminin S		ns	***	**
34	phenanthrene	Fcc02471	omega class glutathione S-transferase	VIP	ns	ns	ns
35	phenanthrene	Fcc05124	CCCTC-binding factor-like protein	VIP	ns	ns	ns
36	phenanthrene	Fcc04316	phosphoserine aminotransferase		ns	ns	ns
37	phenanthrene	Fcc06085	ABC-type multidrug transport system	VIP	ns	***	***
38	phenanthrene	Fcc03183	scribbled		ns	ns	ns
39	phenanthrene	Fcc01177	ABC transporter ABCC1	VIP	**	***	**
40	phenanthrene	Fcc01609	HSP70; HSP68		**	***	ns
41	phenanthrene	Fcc00015	CYP9/6	VIP	ns	***	***
42	phenanthrene	Fcc00021	Moxd1 protein	VIP	**	***	**
43	phenanthrene	Fcc00058	CYP6N3v2	VIP	ns	ns	ns
44	phenanthrene	Fcc00390	CYP2P3 (fish)		ns	ns	ns

¹ Collembase IDs can be found on http://www.collembase.org
² Important variable in partial least squares differential analysis (VIP); significance levels in two-way ANOVA followed by Bonferroni post hoc testing: ns P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001
³ significance levels in two-way ANOVA followed by Bonferroni post hoc testing: ns P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001

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ISSN: 1471-2199

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