Figure 3
From: Essential role for ALCAM gene silencing in megakaryocytic differentiation of K562 cells
Ectopic expression of ALCAM clusters K562 cells. A) Western blot analysis shows expression of ALCAM in K562 clones stably transfected with ALCAM-GFP (#1, #2), but not in clones transfected with empty vector (#15, #16). B) Live-cell imaging of K562-ALCAM (#1) and control K562 (#15) clones. C) Photomicrographs of K562-ALCAM cells seeded with anti-ALCAM anti-body or IgG and cultured for 24 hours. Note formation of a large aggregate of cells in cultures containing IgG. D) Photomicrographs of pre-formed K562-ALCAM cell clusters treated with anti-ALCAM antibody or IgG for 24 hours. Anti-ALCAM antibody disaggregates cell clusters while IGg has no impact.