Figure 1
From: Essential role for ALCAM gene silencing in megakaryocytic differentiation of K562 cells
ALCAM expression in hematopoietic progenitor cell lines. A) Total RNA was isolated from hematopoietic cells and ALCAM mRNA quantified by quantitative RT-PCR. GAPDH was used as invariant control in the experiment. Data shown is the mean of three analyses each in triplicates. B) Whole cell lysates (15 μg) from indicated cells were blotted for ALCAM protein by western blot analysis and protein loading verified by analyzing the same filters for EF-1α expression.