Figure 3

Disruption of HNF1-alpha and USF recognition sequences decreases UGT1A1 basal expression in HT29 cells. Mutational analysis of the putative HNF1, OCT1, USF and NF-Y binding sites in the UGT1A1 proximal promoter. Relative luciferase activity is expressed as a fold induction over the promoterless pGL3-basic vector (set to value = 1). Columns, mean of triplicates of three independent experiments; bars, SD; *, P < 0.05