Figure 2
Interaction of transcription factors with the UGT1A1 promoter using protein nuclear extracts of UGT1A1-expressing HT29 colon cancer cells. EMSAs were performed with 32P-end-labeled probes. Nuclear protein extracts (5 ÎĽg) from HT29 cells were pre-incubated in the presence of either no competitor or 100-fold molar excess of cold competitor oligonucleotides (C) or consensus sequence (Cs). For supershift assays, 2 ÎĽg of the indicated antibody (Ab) were added directly after the addition of the labeled probe. For USF assays, Ab1 and Ab2 are directed against USF1 and USF2, respectively.