Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]

Table 1 Correlation coefficient between gene expression and shikonins content in arnebia.

Name of the gene	Gene expression in cell culture treated with mevinolin	Gene expression in HSPS
MVA pathway
AeACTH	0.87	0.31
AeHMGS	0.77	0.48
AeHMGR	0.71	0.95
AeMVK	0.62	0.44
AePMVK	0.89	0.28
AeMVDD	0.82	0.20
AeIPPI	0.83	0.04
AeGDPS	0.83	0.39
PP pathway
AePAL	0.83	0.57
AeC4H	0.68	0.59
Ae4-CL	0.73	0.50
Gene for coupling of MVA and PP pathways
AePGT	0.88	0.91

Correlation coefficient was calculated using integrated density value (IDV) of gene expression and the shikonins content in response to the inhibitor (Figure 2) and HSPS (Figure 3), separately. IDV is mentioned in Additional file 7: Supplementary Figure S5 and Additional file 8: Supplementary Figure S6. For Figure 3 linear portion of increase in shikonins content, i.e. day 2, day 4, day 6 and day 8, were considered and accordingly the IDV of gene expression

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