Table 4 Primer sequences, amplicon sizes, and polymerase chain reaction (PCR) amplification efficiency for the candidate reference genes and target gene.

Reference

eEF1A (m)

(F) GGC TGA TTG TGC TGT GCT TA

(R) CTC ACT CCA AGG GTG AAA GC

Reference

eEF1A (h)

(F) ATG TCT GTT GAG CAG CCT TC

(R) GCG GAG TAT ATA AAG GGG TAG C

Reference

eEF1A (s)

(F) CCG TTT TGT CGA GTT TGG T

(R) AGC AAC TGT AAC CGA ACA TAG C

Reference

TBP-1

(F) TGC TTA GTT CCC CTA AGA TAG TGA

(R) CTG AGA CCA AAC ACG ATT TCA

Reference

eIF4A

(F) AAC TCA ACT TGA AGT GTT GGA GTG

(R) AGA TCT GGT CCT GGA AAG AAT ATG

Reference

YT521-B

(F) TGT AGC TTG ATC GCA TAC CC

(R) ACT CCC TGG TAG CCA CCT T

Reference

H3

(F) CAC CAA TGT TCT GCC TAT CG

(R) CAG ACC AAC GAA CAA ACG AC

Reference

E2

(F) CGG TTC TGT GCC AAA ATG T

(R) CAG CTA TCT CCA ACG GTT CA

Target

EF-Tu

(F) AAT GCC CAC CAT GAG AAT TT

(R) ATG CAA GCA AAA CCA CTT GA